Anti-GRASP55 Antibody

Golgi reassembly stacking protein of 55 kDa (also called Golgi reassembly-stacking protein of 55 kDa, GRASP55, or Grs2) is encoded by the gene designated as Gorasp2 in rat & mouse (GORASP2/GRS2/GOLPH6 in human). Golgi stack formation involves two homologous peripheral Golgi proteins, GRASP55 and GRASP65, localized to the medial-trans and cis cisternae, respectively. GRASP55 and GRASP65 stack mammalian Golgi cisternae via a common mechanism of phosphorylation-regulated oligomerization. GRASP55 stacks Golgi membranes by forming oligomers through its N-terminal GRASP domain. This process is regulated by phosphorylation within the C-terminal serine/proline-rich domain. Expression of nonphosphorylatable GRASP55 mutants enhances Golgi stacking in interphase cells and inhibits Golgi disassembly during mitosis. Knockdown of either GRASP55 or GRASP65 by siRNA reduces the number of cisternae per Golgi stack, while simultaneous knockdown of both GRASP proteins leads to disassembly of the entire stack. (PMID 20083603). Cat. No. ABS1520, Anti-GRASP55, is a rabbit polyclonal antiserum raised against a recombinant immunogen encompassing region within the C-terminal half of rat GRASP55.

~54 kDa observed

Epitope: C-terminal

Recombinant protein corresponding to the C-terminal of rat GRASP55.

Anti-GRASP55 Antibody is an antibody against GRASP55 for use in Western Blotting, Immunocytochemistry.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A representative lot detected GRASP55 in HeLa cell lysate (Xiang, Y., and Wang, Y. (2010). J. Cell Biol. 188(2):237-251).
Western Blotting Analysis: A representative lot detected GRASP55 in CHO cells and mouse brain lysate (Joshi, G., et al (2014). PNAS. 111(13):E1230-1239).
Immunocytochemistry Analysis: A representative lot detected GRASP55 in HeLa cells (Xiang, Y., and Wang, Y. (2010). J. Cell Biol. 188(2):237-251).
Immunocytochemistry Analysis: A representative lot detected GRASP55 in CHO cells (Joshi, G., et al (2014). PNAS. 111(13):E1230-1239).

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected GRASP55 in 10 µg of mouse brain tissue lysate.

Concentration: Please refer to lot specific datasheet.

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