Anti-Chromatin-Modifying Protein 2a

Charged multivesicular body protein 2a (UniProt: O43633; also known as Chromatin-modifying protein 2a, CHMP2a, Putative breast adenocarcinoma marker BC-2, Vacuolar protein sorting-associated protein 2-1, Vps2-1, hVps2-1) is encoded by the CHMP2A (also known as BC2, CHMP2) gene (Gene ID: 27243) in human. CHMP2a is a core component of the endosomal sorting required for transport complex III (ESCRT-III) that is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. It localizes to the mid-body of dividing cells. CHMP2a consists of an acidic C-terminus and the basic N-terminus that render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. Cells lacking CHMP2A exhibit mitotic defects. They form transient monopolar spindles and fail to segregate chromosomes normally and also fail to complete abscission. Cell death is also reported to be higher in cells depleted of CHMP2A, CHMP4B, and VPS4B.

~32 kDa observed; 25.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Epitope: unknown

GST-tagged full-length human recombinant Chromatin-modifying protein 2a.

Anti-Chromatin-Modifying Protein 2a, Cat. No. ABS1680, is a rabbit polyclonal antibody that targets Charged multivesicular body protein 2a and has been tesed for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected Chromatin-Modifying Protein 2a in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad Sci USA. 107(29):12889-94; Bajorek, M., et. al. (2009). Nat Struct Mol Biol. 16(7):754-62).

This polyclonal antibody specifically detects human Chromatin-modifying protein 2a.

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Chromatin-Modifying Protein 2a in 10 µg of HEK293 cell lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.