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Merck
CN

ABS45

抗-磷酸-MYPT1（Thr696）抗体

from rabbit, purified by affinity chromatography

别名:

Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗-磷酸-MYPT1（Thr696）抗体, from rabbit, purified by affinity chromatography

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

(~130 kDa observed; 115.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).)

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

canine (based on 100% sequence homology), rat (based on 100% sequence homology), primate (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology), horse (based on 100% sequence homology), Xenopus (based on 100% sequence homology)

technique(s)

inhibition assay: suitable (Peptide Inhibition Assay)
western blot: suitable

NCBI accession no.

NP_001137357.1

UniProt accession no.

O14974

shipped in

wet ice

target post-translational modification

phosphorylation (pThr696)

Quality Level

100

Gene Information

human ... PPP1R12A(4659)

相关类别

Analysis Note

对照
Calyculin/Okadaic处理和未处理的HEK293细胞裂解物
通过蛋白质印迹对 HEK293 细胞裂解液进行了评估。
蛋白质印迹分析：使用0.1 µg/mL该抗体检测10 µg Calyculin/Okadaic处理和未处理的HEK293细胞裂解物在Thr696磷酸化的MYPT1。

Application

抗-磷酸-MYPT1（Thr696）抗体是一种用于WB的抗磷酸MYPT1（Thr696）的抗体。
研究子类别
激酶 & 磷酸酶
研究类别
信号传导
蛋白质印迹（SNAP ID）分析： 0.1 µg/mL先前批次在10 µg NIH/3T3和细胞裂解物中检测到MYPT1。

Biochem/physiol Actions

该兔多克隆抗体可特异性检测苏氨酸696上的MYPT1磷酸化。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

Anti-phospho-MYPT1 （Thr696，货号ABS45）是一种兔多克隆抗体，可检测苏氨酸696上的MYPT1磷酸化，经测试可用于蛋白质印迹和肽抑制分析。
在含 0.1 M Tris-甘氨酸（pH 7.4）150 mM NaCl 和 0.05％ 叠氮化钠的缓冲液中的纯化的兔多克隆抗体
蛋白磷酸酶1调节亚基12A（UniProt: O14974；也称肌球蛋白磷酸酶靶向亚基1，肌球蛋白磷酸酶靶标亚基1，蛋白磷酸酶肌球蛋白结合亚基，由PPP1R12A（也称MBS、MYPT1）基因（基因ID：4659）编码。 肌球蛋白轻链磷酸酶及其调节亚基，肌球蛋白磷酸酶靶向亚基1（MYPT1），在肌球蛋白轻链激酶的作用下调节肌球蛋白轻链的Ca2+依赖性磷酸化，该激酶是平滑肌收缩所必需的。 肌球蛋白磷酸酶调节在鸟苷三磷酸酶Rho下游肌动蛋白和肌球蛋白的相互作用，其通过Rho激酶的作用抑制肌球蛋白磷酸酶。MYPT1位于应激纤维上，并分布在细胞膜附近和细胞间接触处，以调节肌球蛋白磷酸酶的活性。它是在ROCK1和CDC42BP的共同作用下在Thr-696上磷酸化，Thr-696通过分子内机制抑制PP1C 活性。PPP1R12A 的突变与泌尿生殖和/或脑畸形综合征（GUBS）有关，该综合征是一种常染色体显性综合征，特征是多种先天性异常，包括泌尿生殖系统畸形和脑畸形。已经描述了通过选择性剪接产生的MYPT1的五种亚型。（参考文献：Qiao, J., et al. (2014)。J. Biol. Chem. 289(32):22512-22523）。

Immunogen

KLH偶联的线性肽，对应于人MYPT的磷酸苏氨酸696周围的11个氨基酸。
表位：磷酸化Thr696

Other Notes

替代品：07-251
浓度：请参考批次特异性浓缩物的分析证书。

Physical form

亲和纯化
纯化的兔多克隆抗体，含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠。

Preparation Note

自收到之日起，在 2-8°C 条件下可稳定保存1年

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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