ABT28
Anti-Autotaxin Antibody
from rabbit, purified by affinity chromatography
别名:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
种属反应性(根据同源性预测)
canine (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
技术
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... ENPP2(5168)
一般描述
Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness.
~100/125 kDa observed.
3 isoforms are produced by alternative splicing with molecular weights of 99, 105, and 101 kDa The calculated molecular weight of this protein is 99 kDa, but can be observed at ~125 kDa due to high glycosylation. An uncharacterized band may appear at ~35 kDa in some lysates.
3 isoforms are produced by alternative splicing with molecular weights of 99, 105, and 101 kDa The calculated molecular weight of this protein is 99 kDa, but can be observed at ~125 kDa due to high glycosylation. An uncharacterized band may appear at ~35 kDa in some lysates.
免疫原
Epitope: Substrate binding region
KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region.
应用
Detect Autotaxin using this Anti-Autotaxin Antibody validated for use in WB, IH(P).
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed with Tween.
Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues.
Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues.
生化/生理作用
This antibody recognizes Autotaxin at the substrate binding region.
分析说明
Control
Oligodendrocyte precursor cell lysate
Oligodendrocyte precursor cell lysate
Evaluated by Western Blot in oligodendrocyte precursor cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Paul A Volden et al.
Cancer prevention research (Philadelphia, Pa.), 9(5), 367-378 (2016-02-11)
Lysophosphatidic acid (LPA), acting in an autocrine or paracrine fashion through G protein-coupled receptors, has been implicated in many physiologic and pathologic processes, including cancer. LPA is converted from lysophosphatidylcholine (LPC) by the secreted phospholipase autotaxin (ATX). Although various cell
Filipa L Cardoso et al.
Journal of neuroinflammation, 12, 82-82 (2015-05-01)
The inflammatory mediator lipopolysaccharide (LPS) has been shown to induce acute gliosis in neonatal mice. However, the progressive effects on the murine neurodevelopmental program over the week that follows systemic inflammation are not known. Thus, we investigated the effects of
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