Inversin is a tubulin-associated protein, which is highly expressed in the cilia of renal tubules. It contains sixteen ankyrin repeats at the N-terminus and two IQ domains at the C-terminus. The ankyrin repeats facilitate protein-protein interactions, whereas the IQ domains are involved in calmodulin binding. Previous studies have indicated that inversin modulates the Wnt signaling pathway, and interacts with a plethora of intracellular signaling targets, including Disheveled, Frizzled-8, alpha- and beta catenins, NPHP1, and NPHP3. Inversin is localized to mitotic spindle fibers, and plays an important role in renal development and left-right axis determination. Defects in inversin result in nephronophthisis type 2, an autosomal recessive cystic kidney disease.

~165/12 kDa observed. Uniprot describes 3 isoforms at ~11, ~100, and ~118 kDa The calculated molecular weights are 11 and 118 kDa, however Inversin has been shown as a ~12 and ~165 kDa band in western blots (Nurnberger, J., et al. (2004). J Am Soc Nephrol. 15:1700–1710.). Uncharacterized bands may appear at ~45, ~53, and ~55 kDa

No homology to Rat was found.

KLH-conjugated linear peptide corresponding to human Inversin.

Anti-Inversin Antibody detects level of Inversin & has been published & validated for use in Western Blotting & IHC.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western Blot Analysis: 1 µg/mL from a representative lot detected Inversin in 10 µg of human liver tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Inversin in human kidney tissue.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEK293 cell lysate

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Inversin in 10 µg of HEK293 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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