Apolipoprotein A-II, human

High Density Lipoprotein (HDL) isolated by sequential isopycnic ultracentrifugation are delipidated. Apo A-II is purified by column chromatography in 6M urea (Schonfeld et al. 1977). Purity is determined by quantitative densitometry of SDS polyacrylamide gels (Laemlli 1970). Protein is determined by the Pierce BCA method (which was found to be comparable to the Lowry method) with 1% BSA as the standard. Apo A-II, which is a dimer composed of identical subunits, has a Mr of 17 kD and a pI of 4.9. Apo-II activates hepatic lipase and inhibits LCAT. Apolipoproteins are soluble in aqueous solutions. However, due to its amphipathic structure Apo-II self-associates into oligomers at high protein concentrations. Apo A-II exists as dimers in buffers with 5M guanidine-HCL or 6M urea.

MOLECULAR WEIGHT:

17,400

Product Source: Normal human plasma, fresh, non-frozen. Tested negative for HIV-1, HIV-2, HCV and HBc antibodies and HBsAg.

Liquid in 10 mM NH4HCO3, pH 7.4. Non-sterile, no preservatives.

Maintain lyophilized material at < -20°C for up to six months. Maintain reconstituted Apo A-II at +4°C for up to 4 weeks. Freezing solutions of apolipoproteins in the absence of denaturants may favor increased self-association or aggregation.

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