AP1160
Anti-NDRG1 Mouse mAb (2D7)
liquid, clone 2D7, Calbiochem®
别名:
Anti-N-myc Downstream Regulator 1
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
2D7, monoclonal
Species reactivity:
human
Application:
Citations:
1
产品名称
Anti-NDRG1 Mouse mAb (2D7), liquid, clone 2D7, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
2D7, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (0.7 µg/mL))
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NDRG1(10397)
Analysis Note
Positive Control
Human endometrium tissue, HeLa cells
Human endometrium tissue, HeLa cells
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (0.7 g/ml)
Disclaimer
Toxicity: Regulatory Review (Z)
General description
Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDRG1 protein.
Recognizes the ~40-45 kDa NDRG1 protein in HeLa cells and human endometrium tissue.
This Anti-NDRG1 Mouse mAb (2D7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of NDRG1.
Immunogen
Full-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Jiang, K., et al. 2010. Scand. J. Gastroenterol45, 898.
Leth-Larsen R, et al. 2009. Mol. Cell Proteomics8, 1436.
Leth-Larsen R, et al. 2009. Mol. Cell Proteomics8, 1436.
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Lianghui Dong et al.
PLoS pathogens, 17(6), e1009645-e1009645 (2021-06-03)
The presumed DNA helicase encoded by ORF44 of Kaposi's sarcoma-associated herpesvirus (KSHV) plays a crucial role in unwinding viral double-stranded DNA and initiating DNA replication during lytic reactivation. However, the regulatory mechanism of KSHV ORF44 has not been fully elucidated.
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