biological source
goat
conjugate
TRITC conjugate, rhodamine conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunofluorescence: suitable
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
General description
IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Immunogen
Epitope: IgG γ chain
Prepared from purified mouse IgG γ chain. Pooled antisera from goats hyperimmunized with mouse IgG paraproteins.
Application
Goat anti-Mouse IgG γ chain Antibody, TRITC conjugate, Species Adsorbed detects level of Mouse IgG γ chain & has been published & validated for use in ELISA & IF.
Immunofluorescence: ≤1 μg/106 cells.
ELISA
Optimal working dilutions must be determined by the end user.
ELISA
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Fragment Specific Secondary Antibodies
Secondary Antibodies Adsorbed for Dual Labeling
Fragment Specific Secondary Antibodies
Biochem/physiol Actions
Reacts with heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3. Absorbed for mouse IgM and IgA, pooled human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
The antibody reacts with the heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Physical form
Affinity Chromatography
Purified by affinity chromatography on pooled mouse IgG covalently linked to agarose. Liquid in 1.0 mL PBS/NaN3.
Preparation Note
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. do not freeze.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Guangchen Ji et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(6), 1378-1393 (2016-12-25)
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