biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... WT1(7490)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~47-55 kDa Wilm′s tumor protein.
Recognizes the ~47-55 kDa Wilm′s tumor protein in Wilm′s tumor tissue.
This Anti-Wilm′s Tumor Protein Rabbit pAb is validated for use in Immunoblotting, Paraffin Sections, Immunoprecipitation for the detection of Wilm′s Tumor Protein.
Immunogen
Human
a synthetic peptide corresponding to amino acids from the C-terminus of human Wilm′s tumor protein
Application
Immunoblotting (see comments)
Paraffin Sections (1:100, heat pre-treatment required)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, 0.2% BSA, pH 7.6.
Preparation Note
Following initial use, aliquot and freeze (-20°C) for long-term storage.
Analysis Note
Positive Control
Wilm′s tumor tissue
Wilm′s tumor tissue
Other Notes
Huff, V. 1998. Am. J. Med. Genet.79, 260.
Staining of formalin-fixed, paraffin-embedded tissues requires antigen retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at room temp for 20 min. This antibody is also reported to work for immunoblotting and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable