CB1024
Anti-ACTN4 Mouse mAb (4D10)
liquid, clone 4D10, Calbiochem®
别名:
Anti-Actinin α4
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
4D10, monoclonal
Species reactivity:
mouse, human, rat
Application:
Citations:
产品名称
Anti-ACTN4 Mouse mAb (4D10), liquid, clone 4D10, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
4D10, monoclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (20 µg/mL)
Paraffin Sections (3 µg/mL))
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTN4(81)
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
PC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue
PC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue
Application
ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (20 g/ml)
Paraffin Sections (3 g/ml)
Disclaimer
Toxicity: Regulatory Review (Z)
General description
Anti-ACTN4, mouse monoclonal, clone 4D10, recognizes the ~90-100 kDa ACTN4 in PC-12, HeLa, Raw 264.7, MCF-7, NIH3T3 cells & in human testes. It is validated for ELISA, WB, ICC, and paraffin sections.
Purified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.
Recognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.
Immunogen
A recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg.39, 61.
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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