Virtually all lymphoid and myeloid cells in bone marrow are reactive with the anti-VLA-4 antibody. VLA-4 is absent or minimally detectable on stromal cells. Myeloid cells maintained in Dexter-type long-term cultures of murine bone marrow are positive, as are lymphoid cells from Whitlock-White cultures.

Anti-Integrin α4 Antibody, clone PS/2 detects level of Integrin α4 & has been published & validated for use in FC, IF, IP, IH.

Flow cytometry: 2 μg per 10⁶ cells

Immunohistochemistry on frozen tissue sections

Immunoprecipitation

Cell adhesion assays

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Clone PS/2 recognises the murine alpha4 chain of the VLA-4 integrin heterodimer, 150 kDa. The antibody also reacts with human VLA-4.

Reactivity with other species has not been confirmed.

Format: Purified

Purified rat IgG2bκ in 0.01 M phosphate buffer, pH 7.1 containing no preservatives.

Maintain at -20ºC for up to 12 months from date of receipt.Upon first use, aliquot into smaller quantities to avoid additional freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1131

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