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Merck
CN

CBL158

Anti-Integrin β2 [CD18] Antibody

clone MEM 48, Chemicon®, from mouse

别名:

CD18

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
MEM 48, monoclonal
Application:
FACS, IHC, WB
Citations:
15
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

MEM 48, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGB2(3689)

Immunogen

Leucocytes from an LGL - type leukaemia

Application

Anti-Integrin β2 [CD18] Antibody detects level of Integrin β2 [CD18] & has been published & validated for use in FC, WB, IH.
Immunohistochemistry: Identification of CD18+ leucocytes on cryostat sections

Function Blocking: Studies of cell-cell and cell-matrix adhesion, this antibody will block cell-cell interaction, (Grönholm, 2018)

Flow Cytometry

Western Blot

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Biochem/physiol Actions

This antibody recognizes the CD18 molecule, a 95 kDa integrin molecule (beta 2 integrin) that forms the common beta chain of the CD11/CD18 heterodimer integrins which are expressed on all leucocytes. The beta2 integrin is the ICAM 1 receptor

FUSION PARTNER:

Sp 2/0 - Ag 14 myeloma cell line

Physical form

Format: Purified
The monoclonal is presented in phosphate buffered saline containing 15mM sodium azide, pH 7.4. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Preparation Note

Maintain at 2o-8oC for up to 12 months from date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C for up to one year.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL514

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


分析证书(COA)

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Structure and function of leukocyte integrins.
Larson, R S and Springer, T A
Immunological Reviews, 114, 181-217 (1990)
Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules
Stefanova, I and Horejsi, V
Journal of immunology (Baltimore, Md. : 1950), 147, 1587-1592 (1991)
Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein.
Bazil, V, et al.
Folia Biologica (Praha.), 36, 41-50 (1990)
The role of galectin-3 in phagocytosis of Candida albicans and Candida parapsilosis by human neutrophils.
Linden, JR; Kunkel, D; Laforce-Nesbitt, SS; Bliss, JM
Cellular Microbiology null
Amir E Zeituni et al.
Journal of immunology (Baltimore, Md. : 1950), 183(9), 5694-5704 (2009-10-16)
The oral mucosal pathogen Porphyromonas gingivalis expresses at least two adhesins: the 67-kDa mfa-1 (minor) fimbriae and the 41-kDa fimA (major) fimbriae. In periodontal disease, P. gingivalis associates in situ with dermal dendritic cells (DCs), many of which express DC-SIGN

全球贸易项目编号

货号GTIN
CBL15804053252742859

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