Thyroglobulin conjugated synthetic peptide of extreme carboxy terminus of human keratin 14 (last 15 residues)

Research Category
Cell Structure

Research Sub Category
Cytokeratins

Suitable for staining formalin fixed wax sections or frozen tissues. In order to obtain 100% consistency in staining it is considered essential to either microwave treat fixed tissue sections or alternatively use the more recently recommended high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use with CBL 197 is neutral buffered formalin.

Optimal working dilutions must be determined by the end user.

This Anti-Cytokeratin 14 Antibody, clone LL002, FITC conjugated is validated for use in IH(P) for the detection of Cytokeratin 14.

Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cell types from simple epithelial cell types. Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.

FUSION PARTNER: NS1 myeloma cell line

The conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1 mg/mL bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.

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