CC076
人IV型胶原蛋白
from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation
登录并加购，请在购物车里查看协议价、货期和发货地。
选择规格
变更视图
关于此项目
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
human
Form:
liquid, solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
1 mg/mL
Mol wt:
300 kDa
Assay:
95% (Human collagen type IV, SDS-PAGE)
产品名称
人IV型胶原蛋白, 1 mg/mL, solution, BioReagent, suitable for cell culture
biological source
human
Quality Segment
assay
95% (Human collagen type IV, SDS-PAGE)
form
liquid, solution
mol wt
300 kDa
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
impurities
<5% Human collagen types I-III, V, VI, and non-collagen proteins
input
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
solubility
water: soluble at 20 °C
NCBI accession no.
UniProt accession no.
binding specificity
Peptide Source: Laminin
, Peptide Source: Nidogen
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... COL4A1(1282)
General description
人IV型胶原是皮肤基底膜的主要组成成分。具有天然的三螺旋结构，是由2条α1-样和1条α2-样基因组成的异源三聚体蛋白。4型胶原在多细胞组织中形成折叠状胞外基质，包围特定类型的细胞，比如脂肪细胞、骨骼肌细胞、心肌细胞和平滑肌细胞。 经过胃蛋白酶温和处理后，将人胶原蛋白IV从洗涤过的解剖组织中提取到稀乙酸中。通过差异盐沉淀法纯化4型胶原。纯度和天然螺旋结构的保留通过SDS-PAGE和与抗特定类型胶原单克隆抗体的反应来控制。分子量：300kDa；溶解度：10mg/mL 来源：人胎盘，HBsAg和HIV抗体阴性
Application
Biochem/physiol Actions
人胶原蛋白IV与Alport和Goodpasture综合征有关，也与严重的风湿性疾病和皮肤病有关，如获得性大疱性表皮松解症。
Physical form
液体纯化蛋白。 在100μ L的0.5 M乙酸溶液中，pH 2.5。 无盐，无防腐剂。
Preparation Note
可在-20ºC保存长达12个月。 应避免反复冻/融循环。
Analysis Note
通过形成微纤维的能力证实了天然结构的保留。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
存储类别
12 - Non Combustible Liquids
WGK
WGK 1
闪点 (F)
Not applicable
闪点 (°C)
Not applicable