生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
DCS-6, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, mouse, rat
制造商/商品名称
Calbiochem®
储存条件
do not freeze
dilution
(Flow Cytometry (5 µg/mL)
Frozen Sections
Immunoblotting (1 µg/mL)
Immunofluorescence (5 µg/mL)
Immunoprecipitation
Neutralization Studies
Paraffin Sections (5 µg/mL, heat pre-treatment required))
同位素/亚型
IgG2a
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... CCND1(595)
mouse ... Ccnd1(12443)
rat ... Ccnd1(58919)
一般描述
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.
Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).
免疫原
Human
recombinant human cyclin D1
应用
Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
Immunoprecipitation (see application references)
Neutralization Studies (see application references)
Paraffin Sections (5 µg/ml, heat pre-treatment required)
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
Immunoprecipitation (see application references)
Neutralization Studies (see application references)
Paraffin Sections (5 µg/ml, heat pre-treatment required)
包装
Please refer to vial label for lot-specific concentration.
外形
In 50 mM sodium phosphate buffer, 0.2% gelatin.
分析说明
Negative Control
trp E (Ab-1)
trp E (Ab-1)
Positive Control
MCF7, or A431 cells or breast carcinoma tissue
MCF7, or A431 cells or breast carcinoma tissue
其他说明
Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
Inaba, T., et al. 1992. Genomics13, 565.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Shumpei Yamada et al.
International journal of cancer, 111(1), 17-22 (2004-06-09)
Cyclin E and Cdk2 have been shown to play an important role in G1/S transition of the cell cycle. Two E-type cyclins (E1 and E2) have been identified to date and share functionally similarities. Upregulation of these cyclins has been
Marie-Noëlle Giraud et al.
Journal of applied physiology (Bethesda, Md. : 1985), 99(1), 313-322 (2005-07-23)
Neuregulin/ErbB2-induced kinase signaling provides essential survival and protection clues for functional integrity of the adult heart and skeletal muscle. To define the regulatory pathways involved in neuregulin-dependent muscle cell survival, we set out to map the largely unknown transcript targets
Banumathi K Cole et al.
Molecular and cellular biology, 28(4), 1274-1284 (2007-12-19)
Merlin, the product of the NF2 tumor suppressor gene, is closely related to the ERM (ezrin, radixin, moesin) proteins, which provide anchorage between membrane proteins and the underlying cortical cytoskeleton; all four proteins are members of the band 4.1 superfamily.
Raanan Berger et al.
Cancer research, 66(11), 5723-5728 (2006-06-03)
The mechanisms underlying the progression of prostate cancer to a state of resistance to hormone ablation remain poorly understood. Here, we have investigated the relationship between androgen receptor (AR) and Her-2/neu in prostate cancer cells. Overexpression of Her-2/neu (c-ErbB2) activates
Yanyuan Wu et al.
Cancer research, 70(13), 5475-5485 (2010-06-17)
Trastuzumab treatment has improved the overall survival of HER2-overexpressing breast cancer patients. However, many of these patients will eventually become resistant to treatment. The mechanisms that contribute to resistance to trastuzumab are unknown. In this study, we tested the hypothesis
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