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Merck
CN

CCYTMG-90K-PX13

Millipore

MILLIPLEX® Canine Cytokine/Chemokine Magnetic Bead Panel - Premixed 13 Plex - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

别名:

canine interleukin immunoassay panel, luminex canine cytokine chemokine growth factor multiplex assay, luminex canine pro inflammatory anti inflammatory cytokine panel, millipore canine inflammation multiplex kit

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UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.47
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质量水平

种属反应性

canine

制造商/商品名称

Milliplex®

assay range

accuracy: 91-109%
sensitivity: 13.6 pg/mL
(IFN-γ)

sensitivity: 21.0 pg/mL
(MCP-1)

sensitivity: 21.7 pg/mL
(IL-8)

sensitivity: 3.2 pg/mL
(IP-10)

sensitivity: 3.5 pg/mL
(IL-2)

sensitivity: 3.7 pg/mL
(IL-6)

sensitivity: 5.3 pg/mL
(KC-like)

sensitivity: 5.8 pg/mL
(IL-18)

sensitivity: 6.1 pg/mL
(TNFα)

sensitivity: 7.5 pg/mL
(IL-7)

sensitivity: 8.5 pg/mL
(IL-10)

sensitivity: 9.0 pg/mL
(IL-15)

sensitivity: 9.2 pg/mL
(GM-CSF)

standard curve range: 12.2-50,000 pg/mL
(All analytes)

技术

multiplexing: suitable

相容性

configured for Premixed

检测方法

fluorometric (Luminex xMAP)

运输

wet ice

一般描述

In addition to studying inflammation for dog nutrition and disease research, canine animal models are important for human biomedical research and drug development. Cytokine and chemokines are molecular mediators of immune cell response in inflammation and immunity. Quantification of cytokines and chemokines is essential to understanding the immune system, as well as for researching disease states such as autoimmune, allergic reactions, sepsis, and cancer.

The MILLIPLEX® Canine Cytokine Panel is 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine serum, plasma, tissue/cell lysate and culture supernatant samples: GM-CSF, IFNγ, KC-like, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, MCP-1, and TNF-a. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

应用

  • Analytes: GM-CSF, IFNγ, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, KC-like, MCP-1, and TNF-α.
  • Recommended Sample Type: Canine serum, plasma, cell culture supernatant and tissue/cell lysates
  • Recommended Sample Dilution: 25 μL of a 1:3 dilution of plasma or serum, or 25 μL cell culture supernatant
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25° C)
  • Research Category: Inflammation & Immunology

生化/生理作用

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

包装

Everything you need in a single kit.

制备说明

Recommended storage for kit components is 2 - 8°C

其他说明

Please contact Technical Service for linearity of dilution.

法律信息

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

警示用语:

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

靶器官

Respiratory Tract

储存分类代码

10 - Combustible liquids

法规信息

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Line-Alice Lecru et al.
Animals : an open access journal from MDPI, 12(5) (2022-03-11)
Cutaneous cytokines and chemokines are involved in the pathogenesis of human and canine atopic dermatitis. The aim of the present study was to discriminate cytokine expression in the ear canals of atopic dogs with otitis, dogs with non-allergic inflammatory otitis
Lauren Ruger et al.
IEEE transactions on bio-medical engineering, PP (2022-08-26)
Histotripsy is a non-invasive focused ultrasound therapy that uses controlled acoustic cavitation to mechanically disintegrate tissue. To date, there are no reports investigating histotripsy for the treatment of soft tissue sarcoma (STS). This study aimed to investigate the in vivo
Fabien Moog et al.
Veterinary sciences, 9(4) (2022-04-22)
Erythemato-ceruminous otitis externa (ECOE) is the most common type of otitis in dogs and is generally associated with bacterial and/or yeast infections. The performance of an ear cleaner was assessed over two weeks in canine ECOE, associated with a mild
Vatinee Y Bunya et al.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 33(7), 519-524 (2017-06-27)
The aim of this study was to examine the tolerability of topical tocilizumab eyedrops in normal dogs and to assess whether this preparation alters tear film cytokine levels or conjunctival cytokine mRNA expression. Two percent tocilizumab eyedrops were administered to
Jennifer A Foltz et al.
Frontiers in immunology, 7, 521-521 (2016-12-10)
Canines spontaneously develop many cancers similar to humans - including osteosarcoma, leukemia, and lymphoma - offering the opportunity to study immune therapies in a genetically heterogeneous and immunocompetent environment. However, a lack of antibodies recognizing canine NK cell markers has

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