MILLIPLEX^® Canine Kidney Toxicity Panel

As one of the leading causes of drug candidate attrition, toxicity research is receiving more attention, especially in the search of sensitive, organ-specific toxicity biomarkers. In pharmaceutical and chemical industries, the kidney is routinely assessed during toxicity evaluations. The importance of the kidney as a central detoxification organ leads to a high exposure of renal tissue to drugs, reactive metabolites, and environmental compounds. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. For improved, non-invasive detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and verified for preclinical studies in relevant animal species. The MILLIPLEX^® portfolio provides a valuable research assay to investigate multiple biomarkers of kidney injury in canine urine samples using the Luminex^® xMAP^® instrument platform.

The MILLIPLEX^® Canine Kidney Toxicity Expanded Panel 1 is to be used for the simultaneous quantification of the following 7 analytes in any combination in canine urine samples: Clusterin, Cystatin C, Kidney Injury Molecule-1 (KIM-1), IL-8, Lipocalin-2/NGAL, MCP-1, and Osteopontin (OPN). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls while can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Toxicity

Kidney injury in dogs involves complex inflammatory and cellular responses, reflected in biomarkers such as Clusterin, Cystatin C, IL-8 (CXCL8), KIM-1, MCP-1 (CCL2), NGAL (Lipocalin-2), and Osteopontin (OPN). Clusterin is a stress-induced protein linked to tissue protection and repair. Cystatin C reflects glomerular filtration efficiency. IL-8 and MCP-1 are chemokines involved in immune cell recruitment during inflammation. KIM-1 and NGAL are sensitive indicators of tubular damage, while OPN plays a role in fibrosis and immune modulation. Together, these analytes provide a comprehensive profile of renal injury, inflammation, and recovery in canine models

Sensitivity: See kit protocol for individual analytes sensitivities.

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 7 analytes in canine urine samples.Analytes included: Clusterin, Cystatin C, IL-8 (CXCL8), KIM-1, MCP-1 (CCL2), NGAL (Lipocalin-2), Osteopontin (OPN)Assay Characteristics: Refer to assay protocol for details on cross-reactivity, sensitivity, precision, and accuracy.

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Features:

• Targets key biomarkers of renal injury, inflammation, and tissue remodeling
• Includes 7 analytes covering glomerular filtration, tubular damage, chemokine signaling, and fibrosis
• Multiplex format enables simultaneous detection from minimal sample volume
• Compatible with canine serum, plasma, and urine samples
• Designed for kidney toxicity, nephropathy, and renal recovery research in dogs

Benefits:

• Provides a detailed molecular profile of kidney damage and repair mechanisms
• Supports evaluation of therapeutic interventions targeting renal inflammation and fibrosis
• Facilitates monitoring of acute and chronic kidney injury in preclinical canine models
• Enhances understanding of immune cell recruitment and tubular stress responses
• Streamlines experimental workflows with high-throughput, multi-analyte capability