跳转至内容
Merck
CN

CP01

Anti-Actin (Ab-1) Mouse mAb (JLA20)

liquid, clone JLA20, Calbiochem®

登录 查看组织和合同定价。

选择尺寸

关于此项目

NACRES:
NA.43
UNSPSC Code:
12352203

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

Anti-Actin (Ab-1) Mouse mAb (JLA20), liquid, clone JLA20, Calbiochem®

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

JLA20, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Immunoblotting (1:5000-1:20000)
Immunofluorescence (1:10000,

Recomended dilution for secondary antibody (0.05 µg/mL, 1:2000 dilution))

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ACTB(60)

Other Notes

Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

Analysis Note

Positive Control
All animal cell lines

Application

Immunoblotting (1:5000-1:20000)

Immunofluorescence (1:10000, see application references)



Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

General description

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
  • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
  • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
  • Entrez Gene Name: actin, alpha 1, skeletal muscle
  • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
  • Mu Entrez ID: 11459 ;-; 11465
  • Rat Entrez ID: 29437 ;-; 287876

    • Immunogen

    Chicken
    chicken cytoskeletal proteins

    Physical form

    100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

    Preparation Note

    Following initial thaw, aliquot ascites and freeze (-20°C).

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    未找到合适的产品？  

    试试我们的产品选型工具.

    存储类别

    11 - Combustible Solids

    wgk

    WGK 1

    flash_point_f

    Not applicable

    flash_point_c

    Not applicable

    分析证书(COA)

    输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

    已有该产品？

    在文件库中查找您最近购买产品的文档。

    访问文档库

    Natasa J Stojkov et al.
    Molecular human reproduction, 20(1), 77-88 (2013-07-31)
    The molecular mechanism of stress-associated reproductive dysfunction is complex and largely unknown. This study was designed to systematically analyze molecular effects of systemic in vivo blockade of α1-adrenergic receptors (α1-ADRs) on stress-induced disturbance of cAMP/cGMP signaling in testosterone-producing Leydig cells
    David L Evers et al.
    Antiviral research, 68(3), 124-134 (2005-09-29)
    We report antiviral activity against human cytomegalovirus for certain dietary flavonoids and their likely biochemical mechanisms of action. Nine out of ten evaluated flavonoids blocked HCMV replication at concentrations that were significantly lower than those producing cytotoxicity against growing or
    Valentina Caracciolo et al.
    American journal of physiology. Endocrinology and metabolism, 315(4), E676-E693 (2018-03-07)
    Obesity is associated with adipose tissue inflammation that contributes to insulin resistance. Zinc finger protein 36 (Zfp36) is an mRNA-binding protein that reduces inflammation by binding to cytokine transcripts and promoting their degradation. We hypothesized that myeloid-specific deficiency of Zfp36
    M C Hollander et al.
    Cell cycle (Georgetown, Tex.), 4(5), 704-709 (2005-04-23)
    Gadd45a is a p53-regulated gene whose protein product, like p53, is involved in maintenance of genome stability. Specifically, deletion of Gadd45a leads to extensive aneuploidy as a consequence of centrosome amplification and subsequent abnormal segregation of chromosomes during mitosis. S-phase
    Manuela Martano et al.
    Pathogens (Basel, Switzerland), 9(1) (2020-01-18)
    equine sarcoids are the most frequent skin tumors in equidae worldwide. It is well known that delta bovine papillomaviruses are their causative agents. We have recently shown the presence in equine sarcoids of abnormal vessel structures, which could cause a
    查看所有相关文献

    我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

    联系客户支持