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Merck
CN

CP01

Sigma-Aldrich

Anti-Actin (Ab-1) Mouse mAb (JLA20)

liquid, clone JLA20, Calbiochem®

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关于此项目

UNSPSC代码:
12352203
NACRES:
NA.43

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生物来源

mouse

质量水平

100

抗体形式

ascites fluid

抗体产品类型

primary antibodies

克隆

JLA20, monoclonal

表单

liquid

包含

≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)

种属反应性（根据同源性预测）

all

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

dilution

(Immunoblotting (1:5000-1:20000)
Immunofluorescence (1:10000,

Recomended dilution for secondary antibody (0.05 µg/mL, 1:2000 dilution))

同位素/亚型

IgM

运输

wet ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... ACTB(60)

相关类别

一般描述

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
  • Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1
  • Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN
  • Entrez Gene Name: actin, alpha 1, skeletal muscle
  • Hu Entrez ID: 58, 71 (Related Antibodies: CP64)
  • Mu Entrez ID: 11459 ;-; 11465
  • Rat Entrez ID: 29437 ;-; 287876

    • 免疫原

    Chicken
    chicken cytoskeletal proteins

    应用

    Immunoblotting (1:5000-1:20000)

    Immunofluorescence (1:10000, see application references)



    Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

    外形

    100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

    制备说明

    Following initial thaw, aliquot ascites and freeze (-20°C).

    分析说明

    Positive Control
    All animal cell lines

    其他说明

    Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
    Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
    Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
    Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
    Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

    法律信息

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    免责声明

    Toxicity: Multiple Toxicity Values, refer to MSDS (O)

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    储存分类代码

    11 - Combustible Solids

    WGK

    WGK 1

    闪点(°F)

    Not applicable

    闪点(°C)

    Not applicable

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