biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
monkey, rat, mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer317/pSer317)
Gene Information
human ... CHEK1(1111)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser317.
Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.
This PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
Application
Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)
Physical form
In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preparation Note
Do not aliquot.
Analysis Note
Positive Control
Lysates from COS cells treated with UV
Lysates from COS cells treated with UV
Other Notes
Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol.21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1