生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
8G8, monoclonal
表单
liquid
不包含
preservative
种属反应性
human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (1 µg/mL))
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ENO1(2023)
一般描述
Anti-ENO1, mouse monoclonal, clone 8G8, recognizes the ~45-48 kDa ENO1 in MCF-7 and HeLa cells and in human lymphoma tissue. It is validated for use in ELISA, WB, ICC, & IHC (paraffin sections).
Purified mouse monoclonal antibody. Recognizes the ~45-48 kDa ENO1 protein.
Recognizes the ~45-48 kDa ENO1 protein in MCF-7 and HeLa cells and human lymphoma tissue.
免疫原
Full-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein
应用
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (1 g/ml)
外形
In PBS, pH 7.30.
制备说明
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
MCF-7 cells, Human lymphoma tissue, HeLa cells
MCF-7 cells, Human lymphoma tissue, HeLa cells
其他说明
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Ho, J.A., et al. 2010. Anal. Chem.82 5944.
Zhao, J., et al. 2007. Mol. Cell Proteomics.6 987.
Zhao, J., et al. 2007. Mol. Cell Proteomics.6 987.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Regulatory Review (Z)
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储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
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