biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
8G8, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (1 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ENO1(2023)
General description
Anti-ENO1, mouse monoclonal, clone 8G8, recognizes the ~45-48 kDa ENO1 in MCF-7 and HeLa cells and in human lymphoma tissue. It is validated for use in ELISA, WB, ICC, & IHC (paraffin sections).
Purified mouse monoclonal antibody. Recognizes the ~45-48 kDa ENO1 protein.
Recognizes the ~45-48 kDa ENO1 protein in MCF-7 and HeLa cells and human lymphoma tissue.
Immunogen
Full-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (1 g/ml)
Physical form
In PBS, pH 7.30.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
MCF-7 cells, Human lymphoma tissue, HeLa cells
MCF-7 cells, Human lymphoma tissue, HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Ho, J.A., et al. 2010. Anal. Chem.82 5944.
Zhao, J., et al. 2007. Mol. Cell Proteomics.6 987.
Zhao, J., et al. 2007. Mol. Cell Proteomics.6 987.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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