biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
1E12, monoclonal
form
liquid
does not contain
preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Paraffin Sections (0.5 µg/mL))
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PAX7(5081)
General description
Purified mouse monoclonal antibody. Recognizes the ~60 kDa PAX7 protein.
Recognizes the ~60 kDa PAX7 protein in NIH3T3 cells and human stomach tissue.
This Anti-PAX7 Mouse mAb (1E12) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PAX7.
Immunogen
A recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
Application


ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.5 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
NIH3T3 cells, Human stomach tissue
NIH3T3 cells, Human stomach tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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