Human sE-Selectin-1 ELISA

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FUNCTION: SwissProt: P16581 # Expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. The ligand recognized by ELAM-1 is sialyl-lewis X (alpha(1->3)fucosylated derivatives of polylactosamine that are found at the nonreducing termini of glycolipids).
SIZE: 610 amino acids; 66655 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P16581 ## Belongs to the selectin/LECAM family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 6 Sushi (CCP/SCR) domains.

· 1 aluminum pouches with a MicroWell^™ Plate coated with Monoclonal Antibody (murine) to human E-Selectin · 1 vial (200 μL) HRP-Conjugated Anti-E-Selectin Monoclonal (murine) Antibody · 2 vials (100 ng/mL each, reconstituted) soluble E-selectin Standard, lyophilized {dissolve in 390 microliters for lot 0606032906}. · 1 vial lyophilized control serum · 1 bottle (50 mL) Wash Buffer Concentrate 20X (PBS with 1% TWEEN^® 20)* · 1 vial (5 mL) Assay Buffer Concentrate 20X (PBS with 1% TWEEN^® 20 and 10% BSA)* · 1 bottle (12 mL) Sample Diluent (buffered protein matrix)* · 1 vial (7 mL) Substrate Solution I (tetramethyl-benzidine) · 1 vial (7 mL) Substrate Solution II (0.02 % buffered hydrogen peroxide) · 1 vial (12 mL) Stop Solution (1M Phosphoric Acid) · 1 MicroWell^™ Strip Holder · 2 Adhesive Plate Covers * reagents containing 0.01% thimerosal as preservative

Storage of Kit Components Store kit reagents between 2° and 8°C. Store the lyophilized control at -20°C. Immediately after use remaining reagents should be returned to cold storage (2° to 8°C) with the exception of reconstituted control (-20°C). Expiration date of the kit and reagents is stated on the kit box. Precautions: · Reagents are intended for research use only and are not for use in diagnostic or therapeutic procedures. · Do not mix or substitute reagents with those from other lots or other sources. · Do not use kit reagents beyond expiration date on label. · Do not expose kit reagents to strong light during storage or incubation. · Rubber or disposable latex gloves should be worn while handling kit reagents or specimens. · Some reagents contain thimerosal as preservative, which is highly toxic by inhalation, ingestion, or contact with skin. Thimerosal is a possible mutagen and should be handled accordingly. · Avoid contact of substrate solutions with oxidizing agents and metal. · In order to avoid microbial contamination or cross-contamination of reagents or specimens which may invalidate the test use disposable pipette tips and/or pipettes. · Use clean, dedicated reagent trays for dispensing the conjugate and substrate reagents. · Exposure to acids will inactivate the conjugate. · Glass-distilled water or deionized water must be used for reagent preparation. · Substrate solutions must be at room temperature prior to use.

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