Chemicon Investigator Kits for Integrin Research are a cost-effective source of reagents for all investigators intimately involved with this expanding field of study. The Collagen-Specific Integrins Investigator Kit is designed for research focused on integrins which interact with members of the collagen family of proteins. Collagen-specific integrins include: alpha1, alpha2, and alpha3 subunits, which pair with the integrin beta1 subunit, and alphaV, which pairs with beta3 [Hynes, R. 1992. Cell 69: 11-25].

Integrins belong to a large family of cell surface proteins commonly referred to as glycoproteins. Integrins are composed of noncovalently associated alpha and beta subunits which make up heterodimers: the larger termed alpha chain and the smaller beta chain. At least 16 alpha subunits and 8 beta subunits are expressed, which can give rise to 22 different integrins. Integrins are thought to play critical roles in cell migration, differentiation, and survival. There is now considerable evidence that these transmembrane receptors play an important role in the regulation of gene expression and signal transduction. Excellent reviews of integrin structure, function, and ligand specificity may be found in: Hynes, R. 1992. Cell 69:11-25; Kramer, R. 1993. "Integrin Structure and Ligand Specificity in Cell-Matrix Interactions" in Molecular and Cellular Aspects of Basement Membranes. Academic Press.

Chemicon offers the Collagen-Specific Integrins Investigator Kit for studies of collagen/integrin interactions. All kit reagents are also available in larger sizes. The reagents in this kit are not application specific but rather may be used in a variety of procedures, depending upon the needs of the individual investigator.

alpha1 Mouse Monoclonal Antibody (Part No. MAB1973Z-20) - 1 tube, 20 μg.

alpha2 Mouse Monoclonal Antibody (Part No. MAB1950Z-20)- 1 tube, 20 μg.

alpha3 Mouse Monoclonal Antibody (Part No. MAB2056-20) - 1 tube, 20 μg.

alphaV Mouse Monoclonal Antibody (Part No. MAB1953Z-20)- 1 tube, 20 μg.

beta1 Mouse Monoclonal Antibody (Part No. MAB1951Z-20) - 1 tube, 20 μg.

beta3 Monoclonal Antibody (Part No. MAB1957Z-20) - 1 tube, 20 μg.

alphaVbeta3 Mouse Monoclonal Antibody (Part No. MAB1976Z-20)- 1 tube, 20 μg.

For the study of collagen-associated integrins. With Chemicon′s Collagen Specific Integrins Investigator Kit (ECM425) researchers are provided with 20 μg each of monoclonals to alpha1, alpha2, alpha3, alphaV, alphaVbeta3, beta1, and beta3.

Store antibodies at -20°C.

Important Note: Due to the small volumes contained within the kit, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge before opening to dislodge any liquid entrapped in the container′s cap during shipment.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany