一般描述
CHEMICON体外血管生成检测试剂盒提供方便好用的96孔系统,用于检测内皮细胞的体外成管。在ECMatrix基质胶(Engelbreth-Holm-Swarm/EHS小鼠肉瘤制备的基底膜蛋白凝胶)上培养时,内皮细胞快速对齐并形成中空管状结构。血管生成是一个涉及细胞黏附、迁移、分化和生长的多步骤过程。
ECMatrix基质胶经过特别优化,可最大程度促进成管。如要检测成管的抑制剂或激活剂,只需将内皮细胞悬液与不同浓度的待测抑制剂或激活剂混匀后,加入到ECMatrix基质胶顶部即可。
应用
- 人脐静脉细胞(HUVEC)
- 原代人视网膜微血管细胞
- 血小板衍生生长因子受体-β (PDGFRβ)/PAE细胞
- 牛毛细血管内皮细胞(BCE)
制备说明
其他说明
- ECMatrix(TM)凝胶溶液:(部件号90060)5*1 mL瓶。
- ECMatrix(TM)稀释缓冲液,10X:(部件号90061)1*1 mL瓶。
法律信息
免责声明
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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