General description
Chymostatin. M.W. 604.92
Mixture of types A, B and C. Inhibits Chymotrypsin-like serine proteases including alpha, beta, gamma and delta chymotrtrypsins.
Sequence:
[(S)-1-Carboxy-2-Phenylethyl]-Carbamoyl-a-[2-Iminohexahydro-4(S)-Pyrimidyl]-(S)-Glycyl-X-Phenylalaninal
X: L-Leu (type A), L-Ile (type B), L-Val (type C)
Working concentrations 10-100μM.
Stable for several hours. Soluble in DMSO and glacial acetic acid. Solutions are stable for a month at -20ºC.
5mg/825 microliters in DMSO = 10mM solution.
Dilute 1:100 for 100μM working solution.
Mixture of types A, B and C. Inhibits Chymotrypsin-like serine proteases including alpha, beta, gamma and delta chymotrtrypsins.
Sequence:
[(S)-1-Carboxy-2-Phenylethyl]-Carbamoyl-a-[2-Iminohexahydro-4(S)-Pyrimidyl]-(S)-Glycyl-X-Phenylalaninal
X: L-Leu (type A), L-Ile (type B), L-Val (type C)
Working concentrations 10-100μM.
Stable for several hours. Soluble in DMSO and glacial acetic acid. Solutions are stable for a month at -20ºC.
5mg/825 microliters in DMSO = 10mM solution.
Dilute 1:100 for 100μM working solution.
Biochem/physiol Actions
Inhibitor Type: Protease Inhibitors
Physical form
Lyophilized. Dissolve in dimethylsulfoxide (DMSO). After powder is thoroughly dissolved, dilute the DMSO solution with 0.9% NaCl. The concentration of DMSO should be maintained at 10% or higher. Otherwise chymostatin may precipitate from the solution.
Example: Reconstitute 1 mg of chymostatin with 1.65 mL of DMSO. Then add 14.85 mL of 0.9% NaCl. This furnishes a 10-4 M solution. No preservatives added.
Example: Reconstitute 1 mg of chymostatin with 1.65 mL of DMSO. Then add 14.85 mL of 0.9% NaCl. This furnishes a 10-4 M solution. No preservatives added.
Preparation Note
Maintain lyophilized material at -20ºC for up to one year. Store reconstituted product in aliquots at -20ºC for up to 3-4 weeks. Do not thaw and refreeze.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Chymostatin, a new chymotrypsin inhibitor produced by actinomycetes.
H Umezawa et al.
The Journal of antibiotics, 23(8), 425-427 (1970-08-01)
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