DAB Dilution Buffer 10x Concentrate, for use with ES005

Since first introduced by Graham and Karnovsky (1966), numerous procedures for its use in histochemistry, immunohistochemistry, Western and dot blots have been described (Lewis & Knight, 1977; Larsson, 1988; Gallyas et al., 1982). In the presence of horseradish peroxidase and hydrogen peroxide, DAB oxidatively polymerizes to an insoluble brown polymer. The color can be modified and intensified by treatment with metal salts of silver, copper, nickel, cobalt and osmium (Hsu & Soban, 1982). DAB is a suspected carcinogen and must be handled with caution. CHEMICON supplies DAB as a stable liquid concentrate eliminating the necessity of handling a potentially hazardous material. The stabilization system is unique and prevents formation of partially oxidized DAB excluding nonspecific binding to other heme containing proteins so often observed with powdered DAB preparations. The concentrate can be diluted in appropriate peroxide containing buffers providing the researcher with the capability of formulating any of the numerous published DAB reaction systems. The most common procedure suggests diluting the DAB (Catalog Number ES005) 1:50 in a 100 μmol/L Tris-HCl buffer, pH 7.6, and adding H(2)O(2), to a final concentration of 0.01%. Incubation times range from 5-15 minutes. Prolonged reaction time will result in non-specific staining of other heme proteins.

DILUTION INSTRUCTIONS:

To 9 mL of deionized water add 1 mL of ES010 and 0.1 mL H2O2. This can then be used to dilute ES005. No preservatives added.

TRIS-Citrate buffer containing Hydrogen Peroxide and 0.2% Tween.

Maintain refrigerated at 2-8°C for up to 12 months.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.