Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

16 kDa Calculated

Epitope: methylated lysine 9

KLH-conjugated, synthetic 2X-branched peptide corresponding to histone H3 monomethylated at lysine 9.

Detect monomethyl-Histone H3 (Lys9) using this Milli-Mark Anti-monomethyl-Histone H3 (Lys9)-FITC Antibody validated for use in FC.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Histones

Broad species cross-reactivity is expected based on sequence homology.

Protein A purified

Purified rabbit polyclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
HeLa cells

Evaluated by flow cytometry using HeLa cells.

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