产品名称
Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70, clone MC-813-70, Milli-Mark®, from mouse
biological source
mouse
conjugate
PE
antibody form
purified antibody
antibody product type
primary antibodies
clone
MC-813-70, monoclonal
species reactivity
human
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgG3κ
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FUT4(2526)
General description
The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.
N-terminal GST-tagged, recombinant human MuRF1 full length, expressed in E.coli. Purified using glutathione sepharose.
Immunogen
Human embryonal carcinoma cell line 2102Ep.
Physical form
Protein A purified
Purified mouse monoclonal IgG3 conjugated to PE fluorochrome in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Preparation Note
Maintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Pluripotent human embryonic stem (ES) cells
Pluripotent human embryonic stem (ES) cells
Application
Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugate (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Immunocytochemical staining of fixed H9 human ES cells incubated for 2 hours at 2-8oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugated (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Immunocytochemical staining of fixed H9 human ES cells incubated for 2 hours at 2-8oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugated (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70 is validated for use in FC, IC for the detection of SSEA-4.
Biochem/physiol Actions
This antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In ctontrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In ctontrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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