产品名称
Anti-SSEA-1 Antibody, clone MC-480, PE conjugate, clone MC-480, from mouse, PE
biological source
mouse
conjugate
PE
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MC-480, monoclonal
species reactivity
rat, mouse, human
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FUT4(2526)
Analysis Note
Control
Mouse embryonic Stem Cells
Mouse embryonic Stem Cells
Evaluated by Flow Cytometry with mouse embryonic stem cells.
Application
Anti-SSEA-1 Antibody, clone MC-480, PE conjugate is an antibody against SSEA-1 for use in IH, IP, IF, FC.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Biochem/physiol Actions
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
220 kDa Calculated
SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
Immunogen
Epitope: MC-480
F9 tetracarcinoma stem cells (X-irradiated).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Physical form
Purified mouse monoclonal IgM conjugated to PE fluorochrome in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Preparation Note
Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice.
Chang, BS; Choi, YJ; Kim, JH
Journal of Reproduction and Development null
Pax6- and Six3-mediated induction of lens cell fate in mouse and human ES cells.
Anchan, RM; Lachke, SA; Gerami-Naini, B; Lindsey, J; Ng, N; Naber, C; Nickerson et al.
Testing null
Lei Zhai et al.
PloS one, 12(2), e0172420-e0172420 (2017-02-18)
Embryonic stem cells (ESCs) are pluripotent cells and have the capability for differentiation into any of the three embryonic germ layers. The Wnt/β-Catenin pathway has been shown to play an essential role in ESC differentiation regulation. Activation of β-Catenin by
Xinyu Liu et al.
Cell division, 15(1), 12-12 (2020-12-10)
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) has opened new therapeutic possibilities. However, karyotypic abnormalities detected in iPSCs compromised their utility, especially chromosomal aberrations found at early passages raised serious safety concerns. The mechanism underlying the chromosomal abnormality
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