Milli-Mark^® Anti-CD117 Antibody, c-kit-PE, clone 104D2

145 kDa (Ashman, 1997; Buhring, 1995)

CD117 is a 145 kDa transmembrane protein which belongs to the class III receptor tyrosine kinase family. The extracellular region consists of five immunoglobulin-like domains where the second and third loops are thought to be involved in ligand binding (Ashman, 1997; Bühring, 1995). CD117 has been shown to be a marker for tissue mast cells, hematopoietic stem cells, and progenitor cells in normal human bone marrow. The majority of CD117-positive marrow cells (50-70%) co-express CD34 and comprise progenitor cells and their precursors of all hematopoietic lineages. Apart from mast cells, CD117 is essentially absent from mature hematopoietic cells. Although CD117 is primarily a marker for non-lymphoid progenitor cells in the hematopoietic cells/tissues, including melanocytes, embryonic brain, and the reproductive system (Ashman, 1997). Anti-CD117, 104D2, was included in the Sixth International Workshop and Conference on Human Leucocyte differentiation Antigens, and studies by different laboratories confirmed its reactivity with CD117 (Ashman, 1997).

MOLM-1 megakaryocytic cell line.

Milli-Mark Anti-CD117 Antibody, c-kit-PE, clone 104D2 detects level of CD117 & has been published & validated for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Antibody recognizes Human CD117 antigen.

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
TF-1 cells

Evaluated by flow cytometry using TF-1 cells

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