FCMAB310A4
Milli-Mark® Mouse IgG1-k, clone MOPC-21, Alexa Fluor™ 488 conjugate
Mouse IgG1-k Monoclonal Antibody control validated for use in Flow Cytometry.
产品名称
Milli-Mark® Mouse IgG1-k, clone MOPC-21, Alexa Fluor™ 488 conjugate, Mouse IgG1-k Monoclonal Antibody control validated for use in Flow Cytometry.
biological source
mouse
conjugate
ALEXA FLUOR™ 488
antibody form
purified immunoglobulin
clone
MOPC-21, monoclonal
species reactivity
human
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
isotype
IgG1κ
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Analysis Note
Evaluated for use as flow cytometry isotype control using Jurkat Cells.
General description
The MOPC-21 tumor line that produces mouse IgG1,k, is a mineral oil induced plasmacytoma. The product is originated and carried intraperitoneally in BALB/c mice. Identity and purity of the immunoglobulin is established by immunoelectrophoresis (IEP) and Ouchterlony Double Diffusion (ODD), prior to conjugation. Electrophoresis of the purified immunoglobulin, followed by diffusion versus anti-mouse whole serum, antimouse IgG1 and anti-mouse kappa chain, results in single arcs of precipitation. By ODD the purified immunoglobulin preparation is non-reactive with antisera to mouse lambda light chain, mouse IgA, IgM, IgG2a, IgG2b, and IgG3. The specificity of staining by monoclonal antibodies to human CD antigens should be verified by establishing the amount of non-specific binding to the target cell population. It is recommended that a non-reactive immunoglobulin of the same isotype, concentration and F/P molar ratio be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Devin Rocks et al.
Cell reports, 42(10), 113187-113187 (2023-10-01)
Early-life stress and ovarian hormones contribute to increased female vulnerability to cocaine addiction. Here, we reveal molecular substrates in the reward area, the nucleus accumbens, through which these female-specific factors affect immediate and conditioning responses to cocaine. We find shared
Devin Rocks et al.
Nature communications, 13(1), 3438-3438 (2022-06-16)
The female mammalian brain exhibits sex hormone-driven plasticity during the reproductive period. Recent evidence implicates chromatin dynamics in gene regulation underlying this plasticity. However, whether ovarian hormones impact higher-order chromatin organization in post-mitotic neurons in vivo is unknown. Here, we
Alexi Nott et al.
Nature protocols, 16(3), 1629-1646 (2021-01-27)
We present a nuclei isolation protocol for genomic and epigenomic interrogation of multiple cell type populations in the human and rodent brain. The nuclei isolation protocol allows cell type-specific profiling of neurons, microglia, oligodendrocytes, and astrocytes, being compatible with fresh
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