产品名称
成纤维细胞生长因子碱性,人重组,无动物, The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells.
biological source
human
Quality Level
assay
>95% (SDS-PAGE)
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
impurities
<0.1 EU/μg Endotoxin (of bFGF)
input
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
NCBI accession no.
UniProt accession no.
General description
Application
Physical form
Preparation Note
一般应用:
快速旋转后,在0.1 M磷酸盐缓冲液(pH值6.8)中复溶至0.1-1.0 mg/mL的浓度。 复溶的bFGF应以工作等分试样保存于-20°C中,可保存长达六个月。 多次冷冻/解冻循环会导致大量活性损失。
对于人ES细胞培养:
快速旋转后,在含有0.5%BSA,1 mM DTT和10%甘油的Dulbecco′s PBS过滤溶液中复溶至10 μg/mL。分装并在-20°C下储存长达6个月。 然后,该溶液可以解冻并稀释至4 ng/mL,用于人ES细胞与饲养层的培养,或稀释至8 ng/mL,以补充小鼠胚胎成纤维细胞调节培养基(用于无饲养层的人ES细胞培养)。
Analysis Note
Legal Information
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存储类别
11 - Combustible Solids
wgk
WGK 3
法规信息
此项目有
实验方案
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
商品
Fibroblast growth factors in cell culture and various growth factors for your research
用于细胞培养的成纤维细胞生长因子,以及用于您的研究的各类生长因子
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GF003AF-100UG | 04053252671197 |
| GF003-AF | 04053252270833 |
| GF003AF-MG | 04053252326578 |