Granulocyte Colony-Stimulating Factor (GCSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNFα and IFNγ. Other cell types including fibroblasts endothelial cells, astrocytes and bone marrow stromal cells can also secrete GCSF after LPS, IL1 or TNFα activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express GCSF constitutively. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.

Product Source: E. coli

Cross Reactivty
Mouse

Research Category
Inflammation & Immunology

Research Sub Category
Growth Factors & Receptors

10 ug

Product is filtered through a 0.2 micron filter before lyophilization.

Recombinant animal free human G-CSF is manufactured using all non-animal reagents

Store at -20°C for up to 6 months from date of receipt.

Bioactivity assay: Determined by its ability to stimulate the proliferation of mouse M-NFS-60 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.