GR01
Anti-EGFR (Ab-1) Mouse mAb (528)
liquid, clone 528, Calbiochem®
别名:
Anti-Epidermal Growth Factor Receptor
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关于此项目
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
528, monoclonal
Species reactivity:
human
Application:
Citations:
14
产品名称
Anti-EGFR (Ab-1) Mouse mAb (528), liquid, clone 528, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
528, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Flow Cytometry (5 µg/mL)
Frozen Sections (2.5 µg/mL)
Immunofluorescence (5 µg/mL)
Immunoprecipitation(1 µg/sample)
Neutralization Studies
Paraffin Sections )
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
Analysis Note
Negative Control
HL-60 cells or human brain
HL-60 cells or human brain
Positive Control
A-431 or normal skin tissue
A-431 or normal skin tissue
Application
Flow Cytometry (5 µg/ml)
Frozen Sections (2.5 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation(1 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (see application references)
Frozen Sections (2.5 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation(1 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (see application references)
Disclaimer
Toxicity: Standard Handling (A)
General description
Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references. Recognizes the ~170 kDa EGF receptor.
Recognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.
Other Notes
Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Inmaculada Bañón-Rodríguez et al.
The EMBO journal, 33(2), 129-145 (2014-01-15)
Establishing the correct orientation of the mitotic spindle is an essential step in epithelial cell division in order to ensure that epithelial tubules form correctly during organ development and regeneration. While recent findings have identified some of the molecular mechanisms
David Cachia et al.
Journal of neuro-oncology, 125(2), 401-410 (2015-09-12)
Gliosarcoma is classified by the World Health Organization as a variant of glioblastoma. These tumors exhibit biphasic histologic and immunophenotypic features, reflecting both glial and mesenchymal differentiation. Gliosarcomas can be further classified into primary (de novo) tumors, and secondary gliosarcomas
F Terzi et al.
The Journal of clinical investigation, 106(2), 225-234 (2000-07-21)
The role of EGF in the evolution of renal lesions after injury is still controversial. To determine whether the EGF expression is beneficial or detrimental, we generated transgenic mice expressing a COOH-terminal-truncated EGF-R under the control of the kidney-specific type
Rozanne Lee et al.
Journal of biomolecular screening, 13(3), 210-217 (2008-03-04)
Monoclonal antibodies (mAb) are not only useful reagents but also represent a promising type of therapeutics due to their high affinity and exquisite specificity for their antigens. A critical step in mAb generation is to identify antigen-specific antibodies. Although enzyme-linked
Adam C Midgley et al.
The Journal of biological chemistry, 288(21), 14824-14838 (2013-04-17)
Fibroblast to myofibroblast differentiation drives effective wound healing and is largely regulated by the cytokine transforming growth factor-β1 (TGF-β1). Myofibroblasts express α-smooth muscle actin and are present in granulation tissue, where they are responsible for wound contraction. Our previous studies
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