产品名称
Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14), lyophilized, clone T56/14, Calbiochem®
Quality Level
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
T56/14, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Flow Cytometry (1-5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (1-2 µg/sample)
Immunoblotting (not recommended))
isotype
IgG1
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... TFRC(7037)
Application
Flow Cytometry (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Immunoblotting (not recommended)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Immunoblotting (not recommended)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with S194/5XXO.BU.1 mouse myeloma cells (see application references). Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor protein.
This Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) is validated for use in FC, IF, IP, Immunoblotting for the detection of Transferrin Receptor (Ab-1).
Immunogen
K562 cells
Other Notes
Schneider, C., et al., 1982. J. Biol. Chem.257, 8516.
Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem.49, 357.
Omary, M.B., et al. 1980. Nature286, 888.
Seligman, P.A., et al. 1979. J. Biol. Chem.254, 9943.
Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem.49, 357.
Omary, M.B., et al. 1980. Nature286, 888.
Seligman, P.A., et al. 1979. J. Biol. Chem.254, 9943.
Preparation Note
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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