生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
EGFR.1, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
请勿与下列物质发生反应
rat, mouse
制造商/商品名称
Calbiochem®
储存条件
do not freeze
dilution
(Flow Cytometry (1-5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (1-2 µg/mL) )
同位素/亚型
IgG2b
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
一般描述
Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.
免疫原
Human
partially purified, human EGFR from A431 cells
应用
Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)
包装
Please refer to vial label for lot-specific concentration.
外形
In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.
分析说明
Negative Control
HL-60 cells
HL-60 cells
Positive Control
A-431 cells
A-431 cells
其他说明
EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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