GR15
Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)
liquid, clone EGFR.1, Calbiochem®
别名:
Anti-Epidermal Growth Factor Receptor
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
EGFR.1, monoclonal
Species reactivity:
human
Application:
—
Citations:
2
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
EGFR.1, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Flow Cytometry (1-5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (1-2 µg/mL) )
isotype
IgG2b
shipped in
wet ice
Quality Level
Gene Information
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
target post-translational modification
unmodified
General description
Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.
Immunogen
Human
partially purified, human EGFR from A431 cells
Application
Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.
Analysis Note
Negative Control
HL-60 cells
HL-60 cells
Positive Control
A-431 cells
A-431 cells
Other Notes
EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Nalo Hamilton et al.
BioMed research international, 2015, 925703-925703 (2015-04-16)
Triple-negative breast cancer (TNBC) occurs in 10-15% of patients yet accounts for almost half of all breast cancer deaths. TNBCs lack expression of estrogen and progesterone receptors and HER-2 overexpression and cannot be treated with current targeted therapies. TNBCs often
Jean-Philippe Grossier et al.
The EMBO journal, 33(1), 35-45 (2013-12-25)
Preferred sites of endocytosis have been observed in various cell types, but whether they occur randomly or are linked to cellular cues is debated. Here, we quantified the sites of endocytosis of transferrin (Tfn) and epidermal growth factor (EGF) in
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