产品名称
Anti-EGFR (Ab-5) Mouse mAb (EGFR.1), lyophilized, clone EGFR.1, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
EGFR.1, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
should not react with
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Flow Cytometry (1-5 µg/mL)
Immunofluorescence (1-5 µg/mL)
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (1-2 µg/mL; )
isotype
IgG2b
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
Analysis Note
Negative Control
HL-60 cells
HL-60 cells
Positive Control
A-431 cells
A-431 cells
Application
Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; see application references)
Disclaimer
Toxicity: Standard Handling (A)
General description
Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Recognizes the ~170 kDa EGF receptor protein in A431 cells.
Immunogen
Human
partially purified, human EGFR from A431 cells
Other Notes
Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Stains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.
Physical form
Lyophilized from a volatile buffer, 100 µg BSA.
Preparation Note
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
Dong Duan et al.
Asian Pacific journal of cancer prevention : APJCP, 13(9), 4255-4261 (2012-11-22)
The present study was conducted to evaluate radioimmunoimaging (RII) and in vivo distribution of mixed antibodies 99mTc-EGFR-mAb and 99mTc-CD44- mAb in nude mice bearing human lung adenocarcinoma xenografts. Single and mixed applications of the two radiolabeled monoclonal antibodies (mAbs) were
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