GR37
Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4)
liquid, clone 9A7γE10.4, Calbiochem®
登录 查看组织和合同定价。
选择尺寸
关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
9A7γE10.4, monoclonal
Species reactivity:
chicken, hamster, mouse, porcine, rat, amphibian, human
Application:
Citations:
5
产品名称
Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4), liquid, clone 9A7γE10.4, Calbiochem®
biological source
rat
antibody form
purified antibody
antibody product type
primary antibodies
clone
9A7γE10.4, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
chicken, hamster, mouse, porcine, rat, amphibian, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1:50-1:100)
Gel Shift
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µL/mg protein lysate)
Paraffin Sections (1:50-1:100, sample pre-treatment required))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... VDR(7421)
Analysis Note
Positive Control
Breast carcinoma tissue (see comments)
Breast carcinoma tissue (see comments)
Application
Frozen Sections (1:50-1:100)
Gel Shift (see comments)
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Gel Shift (see comments)
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with mouse myeloma p3 NS 1/Ag4-1 (NS1) mouse myeloma cells. Recognizes both the occupied and unoccupied forms of vitamin D receptor.
Recognizes both occupied and unoccupied vitamin D receptor in breast carcinomas expressing the vitamin D receptor protein.
This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).
Immunogen
purified, chick intestine vitamin D receptor
Other Notes
Brown, A.J. 1999. Nephrol. Dial. Transplant.14, 11.
Fleet, J.C. 1999. Nutr. Rev.57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res.4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem.46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res.47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 241.
Fleet, J.C. 1999. Nutr. Rev.57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res.4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem.46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res.47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 241.
Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Jae Young So et al.
The Journal of steroid biochemistry and molecular biology, 148, 111-121 (2014-12-30)
Tumor-initiating cells (also known as cancer stem cells) are the subpopulation of cells shown to be responsible for tumor initiation, maintenance and recurrence. In breast cancer, CD44(+)/CD24(-/low) cells were identified as tumor-initiating cells. We previously reported that a Gemini vitamin
Misaki Hirose et al.
Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis, 33(2), 132-142 (2012-10-04)
Vitamin D plays an important role in calcium homeostasis and is used to treat secondary hyperparathyroidism among dialysis patients. The biologic activity of vitamin D and its analogs is mediated by vitamin D receptor (VDR), which is distributed widely throughout
Soraya Gutierrez et al.
The Journal of biological chemistry, 279(42), 43581-43588 (2004-08-10)
Vitamin D receptor (VDR) and Runx2 are key regulators of tissue-specific gene transcription. Using the bone-related osteocalcin (OC) gene, we have previously shown that Runx2 is required for the extensive chromatin remodeling that accompanies gene activation. Here, we have addressed
José L Costa et al.
BMC genomics, 10, 499-499 (2009-10-30)
The active form of Vitamin D, 1,25-dihydroxyvitamin D3 (1,25D), has strong anti-proliferative effects, yet the molecular mechanisms underneath this effect remain unclear. In contrast, the molecular mechanism of 1,25D for the regulation of calcium homeostasis has principally been resolved, demonstrating
Jae Young So et al.
PloS one, 8(1), e54020-e54020 (2013-01-18)
CD44, a transmembrane glycoprotein, is a major receptor for extracellular proteins involved in invasion and metastasis of human cancers. We have previously demonstrated that the novel Gemini vitamin D analog BXL0124 [1α,25-dihydroxy-20R-21(3-hydroxy-3-deuteromethyl-4,4,4-trideuterobutyl)-23-yne-26,27-hexafluro-cholecalciferol] repressed CD44 expression in MCF10DCIS.com basal-like human breast
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持