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Merck
CN

HATF00010

Immobilon® -NC Nitrocellulose Membrane

1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, nitrocellulose-based transfer membrane

别名:

Western blotting membrane, blotting membrane, nitrocellulose transfer membrane, transfer membrane

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关于此项目

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22

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产品名称

Immobilon®-NC转移膜, 1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

material

mixed cellulose esters (MCE) membrane
nitrocellulose membrane
plain filter
white filter

feature

hydrophilic

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

33 cm × 3 m

pore size

0.45 μm pore size

capacity

117 μg/cm2 adsorption capacity (insulin)
 160 μg/cm2 adsorption capacity (BSA)
 259 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with India ink
for use with Ponceau-S red

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

Quality Level

400

相关类别

Application

Immobilon-NC转移膜已用于蛋白质印迹分析。

General description

Immobilon-NC (HATF)膜由混合纤维素酯基质组成。它缺乏表面活性剂，在细胞生长过程中可能会干扰细胞壁的完整性。这种膜推荐用于菌落和菌斑提升。

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame
GHS02

signalword

Danger

hcodes

H228

Hazard Classifications

Flam. Sol. 1

存储类别

4.1B - Flammable solid hazardous materials

wgk

WGK 3

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Yanfang Guo et al.
Minerva endocrinologica, 45(1), 29-35 (2018-12-12)
Vitamin D, a fat-soluble secosteroid, plays a key role in several metabolic diseases like diabetes. Diabetes is becoming a third leading chronic disease in the world, which seriously threatens human health. In the current study, we found the beneficial effects
Yongping Zhou et al.
Cancer cell international, 20, 225-225 (2020-06-17)
Pancreatic cancer is a highly malignant gastrointestinal cancer that can widely metastasize during the early stage of disease, and it is associated with one of the worst prognoses among cancers. In this study, we aimed to investigate the function of
Yunhua Hou et al.
Experimental cell research, 387(2), 111750-111750 (2019-12-02)
Lymphoma, a malignant tumor, is mainly characterized by painless lymph node enlargement and hepatosplenomegaly. At present, lymphoma is mainly treated by radiation, chemical drugs, bone marrow transplantation and surgery. However, due to the high degree of heterogeneity, lymphomas are highly
Jianxin Liu et al.
The Journal of international medical research, 48(6), 300060520925598-300060520925598 (2020-06-13)
To investigate the anti-metastatic effects of Babao Dan (BBD) on gastric cancer (GC) cells (AGS and MGC80-3) and explore the underlying molecular mechanisms by which it inhibits epithelial-mesenchymal transition (EMT). AGS and MGC80-3 cells were treated with BBD. In addition
Kanika Verma et al.
Nature communications, 11(1), 2926-2926 (2020-06-12)
Metabolic changes alter the cellular milieu; can this also change intracellular protein folding? Since proteostasis can modulate mutational buffering, if change in metabolism has the ability to change protein folding, arguably, it should also alter mutational buffering. Here we find
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实验方案

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

相关内容

Western Blotting Tools

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

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