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Merck
CN

HATF00010

Millipore

Immobilon® -NC Nitrocellulose Membrane

1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, nitrocellulose-based transfer membrane

别名:

Western blotting membrane, blotting membrane, nitrocellulose transfer membrane, transfer membrane

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关于此项目

UNSPSC代码:
41105339
eCl@ss:
32031602
NACRES:
NB.22

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产品名称

Immobilon®-NC转移膜, 1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

物料

mixed cellulose esters (MCE) membrane
nitrocellulose membrane
plain filter
white filter

质量水平

400

特点

hydrophilic

制造商/商品名称

Immobilon®

技术

western blot: suitable

过滤器长度 × 宽度

33 cm × 3 m

孔径

0.45 μm pore size

容量

117 μg/cm2 adsorption capacity (insulin)
 160 μg/cm2 adsorption capacity (BSA)
 259 μg/cm2 adsorption capacity (goat IgG)

相容性

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with India ink
for use with Ponceau-S red

检测方法

chemiluminescent
colorimetric
fluorometric
radioactive

运输

ambient

相关类别

一般描述

Immobilon-NC (HATF)膜由混合纤维素酯基质组成。它缺乏表面活性剂，在细胞生长过程中可能会干扰细胞壁的完整性。这种膜推荐用于菌落和菌斑提升。

应用

Immobilon-NC转移膜已用于蛋白质印迹分析。

法律信息

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Flame
GHS02

警示用语：

Danger

危险声明

H228

危险分类

Flam. Sol. 1

储存分类代码

4.1B - Flammable solid hazardous materials

WGK

WGK 3

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Bing Wang et al.
Oncology letters, 13(5), 3494-3500 (2017-05-23)
The aim of the present study was to gain insight into the molecular mechanism of gefitinib resistance in non-small cell lung cancer (NSCLC), and demonstrate whether long noncoding RNA (lncRNA) expression signatures differ between gefitinib-sensitive PC9 and gefitinib-resistant PC9 (PC9-R)
1H-Indole-3-Carbonyl-Thiazole-4-Carboxylic Acid Methyl Ester Blocked Human Glioma Cell Invasion via Aryl Hydrocarbon Receptor?s Regulation of Cytoskeletal Contraction
Zhao L, et al.
BioMed Research International (2020)
Yunhua Hou et al.
Experimental cell research, 387(2), 111750-111750 (2019-12-02)
Lymphoma, a malignant tumor, is mainly characterized by painless lymph node enlargement and hepatosplenomegaly. At present, lymphoma is mainly treated by radiation, chemical drugs, bone marrow transplantation and surgery. However, due to the high degree of heterogeneity, lymphomas are highly
Peiying Tian et al.
Cell biology international, 44(8), 1681-1690 (2020-04-14)
Ghrelin-O-acyltransferase (GOAT) is a membrane-bound enzyme that attaches eight-carbon octanoate to a serine residue in ghrelin and thereby acylates inactive ghrelin to produce active ghrelin. In this study, we investigated the function of GOAT in the intestinal mucosal barrier. The
Kanika Verma et al.
Nature communications, 11(1), 2926-2926 (2020-06-12)
Metabolic changes alter the cellular milieu; can this also change intracellular protein folding? Since proteostasis can modulate mutational buffering, if change in metabolism has the ability to change protein folding, arguably, it should also alter mutational buffering. Here we find
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实验方案

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

相关内容

Western Blotting Tools

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

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