质量水平
种属反应性
human
制造商/商品名称
Milliplex®
assay range
accuracy: 89%
(IGF-II)
sensitivity: 19 pg/mL
(MinDC+2SD; IGF-I)
sensitivity: 919 pg/mL
(MinDC+2SD; IGF-II)
standard curve range: 41-30,000 pg/mL
(IGF-I)
standard curve range: 892-650,000 pg/mL
(IGF-II)
inter-assay cv: <15%
intra-assay cv: <10%
(IGF-I)
inter-assay cv: <15%
intra-assay cv: <10%
(IGF-II)
技术
multiplexing: suitable
UniProt登记号
检测方法
fluorometric (Luminex xMAP)
运输
wet ice
基因信息
human ... IGF1(3479)
一般描述
Insulin-like Growth Factors I and II are polypeptide, endocrine hormones similar in molecular structure to insulin. Both IGF-I and IGF-II are produced primarily by the liver and their bio-availabilities are regulated by the IGF binding proteins (IGFBPs).
MILLIPLEX® Human IGF-I, II Panel is a 2-plex kit to be used for the quantitative determination of IGF-I and/or IGF-II in human serum, plasma, tissue/cell extracts, or culture media samples. Reagents are included for the extraction of serum and plasma samples resulting in a 1:60 final dilution. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
MILLIPLEX® Human IGF-I, II Panel is a 2-plex kit to be used for the quantitative determination of IGF-I and/or IGF-II in human serum, plasma, tissue/cell extracts, or culture media samples. Reagents are included for the extraction of serum and plasma samples resulting in a 1:60 final dilution. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
应用
- Analytes: IGF-I, IGF-II
- Recommended Sample Type: Human serum, plasma, cell/tissue culture supernatants or lysates
- Recommended Sample Dilution: Use 25 μL per well of the final 1:60 extracted serum or plasma; Cell/tissue culture supernatants or lysates may require a dilution in appropriate control medium.
- NOTE: Sample extraction, using the reagents included in the kit, is required for serum and plasma samples. The final dilution for extracted serum or plasma samples is 1:60.
- Assay Run Time: Overnight (16-20 hours) at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Metabolic Disorders
生化/生理作用
Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
特点和优势
Design your multiplex kit by choosing available analytes within this panel.
包装
Everything you need in a single kit.
96 well plate
制备说明
Recommended storage for kit components is 2 - 8°C.
其他说明
Please contact Technical Service for linearity of dilution.
Standard curve range: Refer to analysis sheet for exact standard concentracions.
法律信息
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语：
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Sens. 1
储存分类代码
3 - Flammable liquids
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