Quality Segment
feature
accuracy 103–105%
species reactivity
human
description
ED<sub>80</sub> = 0.9 ± 0.1 ng/ml ED<sub>50</sub> = 4.5 ± 0.4 ng/ml ED<sub>20</sub> = 22.2 ± 2.8 ng/ml , ED<sub>80</sub> = 1.2 ± 0.1 ng/ml ED<sub>50</sub> = 5.1 ± 0.4 ng/ml ED<sub>20</sub> = 23.7 ± 2.1 ng/ml , analytes available: Leptin, for protein analysis, 0.5-100 ng/mL (standard curve range)
technique(s)
radioimmunoassay: suitable
UniProt accession no.
detection method
radioactive
General description
FUNCTION: SwissProt: P41159 # May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
SIZE: 167 amino acids; 18641 Da
SUBUNIT: Interacts with SIGLEC6.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].
SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.
SIZE: 167 amino acids; 18641 Da
SUBUNIT: Interacts with SIGLEC6.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].
SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.
Application
Overnight, equilibrium incubation at 4°C. Sample volume 100 μL serum, plasma, or tissue culture media.
Analysis Note
Linearity 88–101%
Disclaimer
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