种属反应性
human
质量水平
制造商/商品名称
Milliplex®
assay range
accuracy: 129%
(Spike Recovery for Melatonin)
sensitivity: 4.3 pg/mL
(Melatonin; MinDC+2SD)
sensitivity: 686 pg/mL
(Cortisol; MinDC+2SD)
standard curve range: 3.4-2,500 pg/mL
(Melatonin)
standard curve range: 686-500,000 pg/mL
(Cortisol)
inter-assay cv: <25%
intra-assay cv: <15%
技术
multiplexing: suitable
检测方法
fluorometric (Luminex xMAP)
运输
wet ice
一般描述
The MILLIPLEX® Human Circadian/Stress Bead Panel enables you to focus on the relevance and therapeutic potential of Melatonin and Cortisol in your research model. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
The MILLIPLEX® Human Circadian/Stress Bead Panel is to be used for the simultaneous quantification of Cortisol and Melatonin. This is a configurable assay, and either or both analytes can be selected. This kit may be used for the analysis of human serum, plasma, tissue/cell lysate, culture supernatant samples, or cerebrospinal fluid (CSF). Serum or plasma samples should be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with this kit.
Panel Type: Neuroscience
The MILLIPLEX® Human Circadian/Stress Bead Panel is to be used for the simultaneous quantification of Cortisol and Melatonin. This is a configurable assay, and either or both analytes can be selected. This kit may be used for the analysis of human serum, plasma, tissue/cell lysate, culture supernatant samples, or cerebrospinal fluid (CSF). Serum or plasma samples should be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with this kit.
Panel Type: Neuroscience
特异性
Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
应用
- Analytes: Cortisol, Melatonin
- Recommended Sample type: serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples
- NOTE: Sample extraction protocols are required for serum/plasma samples. Other sample types (e.g. CSF, culture supernatant, saliva, etc.) do not require sample extraction.
- Recommended Sample dilution: 1:4 for extracted serum and plasma or neat for CSF and culture supernatant
- Assay Run Time: Overnight
- Research Category: Neuroscience Metabolism
- Research Subcategory: Category Metabolic Disorders Obesity Inflammation & Autoimmune Mechanisms
- NOTE: This is a competitive assay
特点和优势
Design your multiplex kit by choosing available analytes within this panel.
包装
Everything you need in a single kit.
96-well plate
组分
Human Circadian/Stress Standard, 1 vial, lyophilized
Human Circadian/Stress Quality Control 1, 1 vial, lyophilized
Human Circadian/Stress Quality Control 2, 1 vial, lyophilized
Assay Buffer, 2 bottles, 30 mL ea
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Human Circadian/Stress Primary Antibodies, 1 bottle, 3.5 mL
Human Circadian/Stress Detection Antibodies, 1 bottle, 5.5 mL
Human Circadian/Stress Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
Human Circadian/Stress Quality Control 1, 1 vial, lyophilized
Human Circadian/Stress Quality Control 2, 1 vial, lyophilized
Assay Buffer, 2 bottles, 30 mL ea
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Human Circadian/Stress Primary Antibodies, 1 bottle, 3.5 mL
Human Circadian/Stress Detection Antibodies, 1 bottle, 5.5 mL
Human Circadian/Stress Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
储存及稳定性
Recommended storage for kit components is 2 - 8°C.
法律信息
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语:
Danger
危险分类
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
储存分类代码
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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