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Merck
CN

HSTCMAG28PMX13BK

MILLIPLEX® Human High Sensitivity T Cell Panel

Bulk (Space Saver) Premixed 96-well Human High Sensitivity T Cell 13-Plex Panel

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UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

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species reactivity

human

Quality Level

200

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

assay range

sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC), standard curve range: 0.18-750 pg/mL
(IL-6), standard curve range: 0.24-1,000 pg/mL
(IL-13), standard curve range: 0.31-1,250 pg/mL
(IL-8), standard curve range: 0.37-1,500 pg/mL
(IL-7), standard curve range: 0.45-1,750 pg/mL
(TNFα), standard curve range: 0.49-2,000 pg/mL
(IL-1β), standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70)), standard curve range: 0.49-2,000 pg/mL
(IL-2), standard curve range: 0.49-2,000 pg/mL
(IL-5), standard curve range: 0.61-2,500 pg/mL
(IFNγ), standard curve range: 1.22-5,000 pg/mL
(GM-CSF), standard curve range: 1.46-6,000 pg/mL
(IL-10), standard curve range: 1.83-7,500 pg/mL
(IL-4), inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

input

cell culture supernatant
serum

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

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General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 13 analytes in human serum, plasma, and cell culture supernatants.

Analytes included: GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, TNF-α

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

  • High Sensitivity Detection: Detects cytokines at picogram levels (as low as 0.11 pg/mL), ideal for low-abundance biomarkers in cell culture supernatants.
  • Robust Data Quality: High accuracy and low variability ensure confidence in results.
  • Pro- vs. Anti-Inflammatory Balance: Simultaneously profile TNF-α (pro-inflammatory) and IL-10 (anti-inflammatory) to evaluate immune equilibrium shifts in sepsis, autoimmune disease, or aging.
  • Low Sample Volume Requirement: Requires only 25 µL of sample per well, preserving rare or limited-volume specimens.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

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Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

存储类别

10 - Combustible liquids

wgk

WGK 3

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Sascha N Goonewardena et al.
Cardiovascular drugs and therapy (2021-11-19)
Vascular and immune dysfunction are hallmarks of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections and coronavirus disease 2019 (COVID-19). Although our understanding of the pathogenesis of COVID-19 has rapidly evolved, much of the focus has been on the immune mechanisms
Amparo Alfonso et al.
Scientific reports, 9(1), 10576-10576 (2019-07-25)
Cyclophilins (Cyps) are ubiquitous proteins that belong to the immunophilins family consistently associated with inflammatory and cardiovascular diseases. While levels of CypA have been extensively studied, less data are available for other Cyps. The purpose of this case-control study was
Jeremias Bayon et al.
Cardiology research, 11(5), 319-327 (2020-08-28)
Cyclophilins (Cyps) are a family of peptidyl-prolyl cis/trans isomerases consistently involved in cardiovascular diseases through the inflammation pathway. This study aims to investigate the serum levels of Cyps (CypA, CypB, CypC and CypD) in patients with coronary artery disease (CAD)

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