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Merck
CN

HSTCMAG28PMX21BK

MILLIPLEX® Human High Sensitivity T Cell Panel

Bulk (Space Saver) Premixed 96-well Human High Sensitivity T Cell 21-Plex Panel

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UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

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species reactivity

human

Quality Level

200

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

assay range

sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC), standard curve range: 0.18-750 pg/mL
(IL-6), standard curve range: 0.24-1,000 pg/mL
(IL-13), standard curve range: 0.24-1,000 pg/mL
(IL-21), standard curve range: 0.31-1,250 pg/mL
(IL-8), standard curve range: 0.31-1,250 pg/mL
(MIP-1α), standard curve range: 0.37-1,500 pg/mL
(IL-7), standard curve range: 0.45-1,750 pg/mL
(TNFα), standard curve range: 0.49-2,000 pg/mL
(IL-1β), standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70)), standard curve range: 0.49-2,000 pg/mL
(IL-2), standard curve range: 0.49-2,000 pg/mL
(IL-5), standard curve range: 0.61-2,500 pg/mL
(IFNγ), standard curve range: 0.61-2,500 pg/mL
(MIP-3α), standard curve range: 0.73-3,000 pg/mL
(IL-17A), standard curve range: 0.92-3,750 pg/mL
(MIP-1β), standard curve range: 1.22-5,000 pg/mL
(GM-CSF), standard curve range: 1.46-6,000 pg/mL
(IL-10), standard curve range: 1.46-6,000 pg/mL
(ITAC), standard curve range: 1.83-7,500 pg/mL
(IL-4), standard curve range: 18.3-75,000 pg/mL
(Fractalkine), standard curve range: 7.93-32,500 pg/mL
(IL-23), inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

input

serum
cell culture supernatant

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

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General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

Application

MILLIPLEX® Qualified assays undergo rigourous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 21 analytes in human serum, plasma, and cell culture supernatants.

Analytes included: Fractalkine/CX3CL1, GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL8/CXCL8, IL-10, IL-12 (p70), IL-13, IL17A/CTLA8, IL-21, IL-23, ITAC/EXCL11, MIP-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20 and TNFα.

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

  • High-Sensitivity T Cell Profiling: Simultaneously quantify 20 key cytokines/chemokines (e.g., IL-17A, IFN-γ, TNF-α) critical for T cell activation, differentiation, and inflammatory responses.
  • Efficient Workflow: Reduces assay time and cost by consolidating multiple ELISAs into one multiplex run.
  • Overnight Incubation Protocol: Enhances sensitivity and signal strength for low-level cytokine detection.
  • Flexible Sample Compatibility: Works with serum, plasma, tissue/cell lysates, and culture supernatants.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

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