Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

CCR8 is a GPCR that binds primarily to the chemokine I-309 (CCL1) (Roos et al., 1997). CCR8 is expressed primarily on Th2 cells although its functional role in T cell recruitment to sites of allergic inflammation is controversial (Chensue et al., 2001; Goya et al., 2003). CCR8+ T-cells are abundant in skin, and rare or absent in the GI tract and peripheral blood, indicating a role in skin-homing of T-cells (Schaerli et al., 2004). In addition, monocyte-derived dendritic cells express CCR8 and utilize CCR8 during mobilization from skin to the lymph node (Qu et al., 2004). Millipore′s cloned human CCR8-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR8 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CCR8.

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

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