Ready-to-Assay M₁ Acetylcholine (Muscarinic) Family Receptor Frozen Cells

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

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Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The muscarinic acetylcholine receptor family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. The M1 receptor, along with the M3 and M5 receptors, signal through Gq/11 and subsequent release of Ca++ from the ER. The M1 receptor is expressed in ganglia and mediates depolarization of ganglia by inhibition of voltage-gated M-type K+ channels. In addition, the M1 receptor mediates venous contraction (Caulfield and Birdsall, 1998). Millipore′s cloned human M1 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant M1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at M1.