Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The melanocortin system consists of five seven-transmembrane spanning G-protein coupled receptors, MC1-MC5 (Gantz and Fong, 2003). Among the five members of the melanocortin receptor family, MC2 and MC5 are expressed in peripheral tissues. The MC2 receptor (ACTH receptor) is almost exclusively expressed in the adrenal cortex whereas MC5 is expressed in several organs including the adrenal cortex. Studies have shown that targeted disruption of the MC5R gene produced mice with a severe defect in water repulsion and thermoregulation caused by decreased production of sebaceous lipids (Chen et al., 1997). In addition, MC5 is required for secretion from several exocrine glands, indicating that melanocortin peptides integrate the function of exocrine glands (Entwistle et al., 1990). Millipore′s cloned human MC5 -expressing cell line is made in the Chem-10 host, which supports high levels of recombinant MC5 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MC5.

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

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