Ready-to-Assay NK3 Tachykinin Receptor Frozen Cells

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

NK3 is one of three mammalian G-protein coupled receptors subtypes (NK1, NK2, NK3) that mediate the biological effects of the tachykinin peptide family. Neurokinin B is the tachykinin family peptide with the highest affinity to NK3R, but it still induces a response with NK1, and NK2 with lower potency (Pennefather et al., 2004; Regoli et al., 1994; Maggi et al., 1993). NK3, like the other tachykinin receptors, signals through Gq to stimulate intracellular calcium (Pinnock et al., 1994). Mammalian tachykinins are present in the brain and gut. NK3 receptor antagonists reduce intestinal motility and nociception in animal models of gastrointestinal disorders, and may be effective in irritable bowel syndrome (Sanger 2004). Millipore′s cloned human NK3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant NK3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to enhance coupling the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between NK3 and its ligands.

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

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