Ready-to-Assay P2Y4 Purinergic Receptor Frozen Cells

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The P2Y GPCRs serve as receptors for extracellular nucleotides. The P2Y4 receptor is activated by UTP, and also by ATP in rodents, and couples to Gq to increase intracellular calcium (von Kügelgen, 2006). P2Y4 is expressed prominently in the intestinal epithelium, where it regulates intestinal chloride secretion. P2Y4 therefore is likely to be involved in infectious diarrhea caused by nucleotides released upon infection with enteropathogenic bacteria (Robaye et al., 2003). Millipore′s cloned human P2Y4-expressing cell line is made in the 1321N1host, which supports high levels of recombinant P2Y4 expression on the cell surface. Thus, the cell line is an ideal tool for screening for agonists and antagonists of P2Y4.

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

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RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.