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IF002

Sigma-Aldrich

干扰素γ蛋白,重组人类

The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.

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eCl@ss:
32160405

生物来源

human

质量水平

检测方案

>98% (SDS-PAGE and HPLC)

比活

≥2 x 10(E7) U/mg

制造商/商品名称

Chemicon®

技术

cell culture | mammalian: suitable

杂质

<0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)

输入

sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)

NCBI登记号

UniProt登记号

运输

dry ice

基因信息

human ... IFNG(3458)

一般描述

产品来源:人,在大肠菌中表达
干扰素-g蛋白(或IFN-γ蛋白)是一种具有强效抗病毒活性的淋巴因子。 它它还可以刺激巨噬细胞和 NK 细胞。 人IFN-γ是一个16.7 kDa的蛋白质,含有143个氨基酸残基。

应用

对于大多数体外应用,IFN-γ在0.1至10 ng/mL的浓度范围内发挥其生物活性。 响应细胞包括(部分列表): 淋巴细胞

联系

替代品:IF3

外形

无菌过滤,从 10 mM 磷酸钠 (pH 7.4) 冻干。

储存及稳定性

冻干 IFN-g尽管在室温下稳定,但最好在-20°C下干燥保存。 快速离心后,用pH 8.0的1x PBS复溶至0.5-1.0 mg/mL的浓度。注意:进一步稀释应配成RPMI+10%FCS或5%HSA在4°C保存一周或在-20°C保存备用。 复溶的材料应以未稀释的等分形式保存于-20°C中,可稳定保存长达六个月。

分析说明

比活度:与标准品相比,人IFN-gamma具有完全的生物活性。通过病毒抗性测定确定的 ED(50) 是≤0.05 ng/mL。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3


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